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KMID : 0380020100250060513
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Korean Journal of Biotechnology and Bioengineering
2010 Volume.25 No. 6 p.513 ~ p.519
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Characterization of Pigment-Producing Kocuria sp. K70 and the Optimal Conditions for Pigment Production and Physical Stability
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Kim Young-Sook
Park Jin-Sook
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Abstract
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Marine bacterium producing pigment was isolated from the solar saltern of Mijo-myeon, Namhae, Korea. Based on phenotypic characteristics and 16S rRNA sequence analysis, the strain was identified as Kocuria sp., which produced a yellow pigment. The pigment showed UV absorption maximum at 469nm. The bacterial strain grew well on Marine broth 2216 culture medium. Productivity of the pigment reached the maximum value after 44 hours at 30℃, 2% NaCl and pH 6.0. The pigment was produced best when supplied by 1% lactose as a carbon source and 1% beef extract as a nitrogen source. The result of the color stability study showed that pigment extracted from the strain by ethanol was stable at -20-25℃ and also showed higher stability over 70% for 14 days in light conditions at 25℃. The pigment extract was also stable for all metal ions tested, except for FeCl2.
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KEYWORD
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bacterial pigment, Kocuria sp., marine bacteria, stability
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